What is a C18 cartridge?

What is a C18 cartridge?

The C18 solid phase extraction (SPE) cartridge uses octadecyl silica as a filler to retain non-polar compounds by strong hydrophobic interaction. The filler retains most of the organic matter and is widely used in the fields of environmental and food safety.

What is a C18 Sep Pak cartridge?

Sep-Pak C18 Vac cartridges contain a hydrophobic, reverse-phase, silica-based bonded phase that is used to adsorb analytes of even weak hydrophobicity from aqueous solutions. The Sep-Pak C18 sorbent is made using the most reproducible bonding technology available, utilizing monofunctional silane chemistry.

How do I choose a SPE cartridge?

The key to choosing a solid phase extraction cartridge is the packing that determines the separation performance in addition to the required specifications. When selecting an extraction column, it is necessary to select a suitable filler according to the type of sample to be tested and its physicochemical properties.

What is a Sep-Pak made of?

Selected Waters Sep-Pak sorbents are available in a unique, multi-capacity, award- winning 96-well plate format designed by Waters. Plates are molded from high-purity polypropylene. Wells are filled with the same high quality sorbents and frit materials available in the respective cartridge formats.

What are SEP Paks?

The Sep-Pak® C18 Cartridge for Column Chromatography is a fantastic, yet inexpensive method of teaching column chromatography. The cartridges are reusable if cleaned and stored properly after use.

What are SEP Paks used for?

Sep-Pak C18 cartridges have found wide application to the extraction, concentration, and fractionation of peptides. Their use has hitherto been limited to stepwise loading, washing, and eluting procedures.

What is a Sep-Pak column?

Column liquid chromatography is used in industry to separate mixtures and detect trace components of a mixture. The Sep-Pak cartridge is filled with a solid nonpolar material that helps to separate components of mixtures. Sep-Pak cartridges are reusable if cleaned and stored properly after use.

What are SPE cartridges?

Solid-Phase Extraction (SPE) is a sample preparation technique that is used to clean up and/or concentrate samples before analysis. Compared to liquid-liquid extraction, SPE is much faster, uses less solvent, and saves money. SPE provides clean extracts and high recoveries.

How do you run a SPE?

To perform normal-phase chromatography with SPE cartridges, use a step gradient of nonpolar solvents with a polar packing material.

1. Condition the cartridge with six to ten hold-up volumes of non-polar solvent, usually the same solvent in which the sample is dissolved.
2. Load the sample solution onto the cartridge bed.